pyrex glass cloning cylinders
general description
Clone a single cell or group of cells by surrounding them with this glass cylinder. Dip the end of the cylinder into sterile silicone grease before pressing to the bottom of a culture flask to create identical cells.cylinders are autoclavable or can be dry heat sterilized.
Material: borosilicate glass cylindrical.
Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.
packing:
Packaged in 60 mm dish with silicone grease on one end